Bull and boar sperm DNA integrity evaluated by sperm chromatin structure assay in the Czech Republic
نویسندگان
چکیده
منابع مشابه
Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA).
Studies over the past two decades have clearly shown that reproductive toxicants cause sperm DNA fragmentation. This DNA fragmentation can usually be detected prior to observing alterations of metaphase chromosomes in embryos. Thus, Sperm Chromatin Structure Assay (SCSA)-detected DNA damage is viewed as the molecular precursor to later gross chromosome damage observed under the light microscope...
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Teratospermia (>60% of morphologically abnormal spermatozoa) is well documented in felids. Even morphologically normal spermatozoa from teratospermic ejaculates have reduced ability to undergo tyrosine phosphorylation, acrosome react, and bind and penetrate oocytes compared with normospermic (<40% abnormal spermatozoa) counterparts. However, it is unknown whether fertilization deficiencies orig...
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Background Morphine as a natural alkaloid (opiate) is considered as one of the problems associated with poor semen production and sperm quality. For the first time, this experimental study was carried out to evaluate the impact of intra-peritoneal injection of morphine on sperm parameters, chromatin quality and DNA integrity of spermatozoa aspirated from cauda epididymis of mice. MaterialsAndMe...
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The sperm chromatin structure assay (SCSA) is a flow cytometric (FCM) technique which exploits the metachromatic properties of Acridine Orange to monitor the susceptibility of sperm chromatin DNA to in-situ acid denaturation. SCSA was used to study the chromatin structure variations of human spermatozoa in semen, both before and after swim-up and after cryopreservation. Semen samples were provi...
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ژورنال
عنوان ژورنال: Veterinární Medicína
سال: 2012
ISSN: 0375-8427,1805-9392
DOI: 10.17221/5668-vetmed